Genetic Control of the Trigger for the G2/m Checkpoint

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چکیده

The current award is divided into two prinicpal components regarding effects stemming from acute low dose radiation. The first is the investigation of the threshold of activation of radiation induced cell cycle arrest and a mechanistic assessment of various radiosensitizing genes known to be involved in this important process. The relevance for this part stems from the prudent application of the Linear No-Threshold model as it informs widely employed radiation protection standards. The second part of the award is the validation of the participation of micro RNA (miRNA) molecules in radiation bystander effect biolgy in which an irradiated cell transmits damage to a non-irradiated bystander cell. This work involved generation of a new technology for isolation and manipulation of individual cells with absolute fidelity to allow for microbeam irradiation of a donor cell and the demonstration that a miRNA molecule is transferred to a particular recipient cell and imparts a biological effect on the recipient. The relevance here is further support of the bystander hypothesis with mechanistic insight as to how damage information is promulgated at the cellular level.

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تاریخ انتشار 2013